Cy5 stain
WebJun 2, 2024 · Allophycocyanin (APC) is a 105 kDa protein, with an extinction coefficient of 700,000 cm -1 M -1 and a quantum yield of 0.68. APC is maximally excited at 652 nm (with a secondary maximum at 625 nm) … WebPE-Cy5 dye is a part of the BD PE family of dyes. This tandem fluorochrome is comprised of a R-Phycoerythrin (PE) donor that has excitation maxima (Ex Max) of 496 nm and 566 …
Cy5 stain
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WebCy5 dye is a traditional far-red–fluorescent label for protein and nucleic acid conjugates used for imaging, flow cytometry, and genomic applications. It is also the basis for the … WebCyanine 5.18 (or Cy5) is a fluorochrome emitting in the long-red/far-red range, usually regarded as unsuitable for direct observation by the human eye. We describe here the optimization of a direct visualization approach to Cy5 labeling, based on a standard fluorescence microscope with mercury light excitation and applicable to both ...
WebNov 19, 2024 · We actually find that PE-Cy5 and PE-Cy5.5 have so much spillover that they should just be avoided altogether (unless you’re using a spectral cytometer). The other … WebAtto 647N is an extraordinary highly fluorescent dye, and Atto 655 are alternatives to Cy5 and Alexa Fluor 647. Atto 633 is an alternative to Alexa Fluor 633. Lectins for carbohydrate binding studies. Primary and Secondary Antibodies for direct and indirect ELISA, Immunoblotting, Immunohistochemistry, and other protein identification applications.
WebPE-Cy5 is a tandem fluorochrome composed of R-phycoerythrin (PE), which is excited by the 488 nm light of an Argon ion laser and serves as an energy donor, coupled to the cyanine dye Cy5, which acts as an energy acceptor and fluoresces at 670 nm. BD Biosciences Pharmingen has maximized the fluorochrome energy transfer in PE-Cy5, … Because they yield brighter and more stable fluorescence, cyanines can advantageously replace conventional dyes such as fluorescein and rhodamines. • Cy3 and Cy5 are the most popular, used typically combined for 2 colors detection. Cy3 fluoresces greenish yellow (~550 nm excitation, ~570 nm emission), while …
WebJul 20, 2024 · Cy5 and Cy5.5 are both cyanine dyes with several differences between them. Used in Fluorescence Resonance Energy Transfer (FRET), Fluorescence Polarization …
WebJan 10, 2024 · Alexa Fluor® dyes are a big group of negatively charged and hydrophilic fluorescent dyes, frequently used in fluorescence microscopy. All the Alexa Fluor® dyes are sulfonated forms of different basic fluorescent substances like fluorescein, coumarin, cyanine or rhodamine (e.g. Alexa Fluor®546, Alexa Fluor®633).The respective laser … cvrtje ribWebFind cy5 and related products for scientific research at MilliporeSigma. US EN. Applications Products Services Support. Advanced Search. Structure Search. Search Within. … dji maverick pro batteryWebCY5 is a CY dye. CY, short for Cyanine, is a compound consisting of two nitrogen atoms connected by an odd number of methyl units. Cyanine compounds have the characteristics of long wavelength, adjustable … cvrle ruma kontaktWebThe dye thus provides more flexibility in multicolor panel design. Vio 515 shows 25% less spillover into the PE channel and facilitates optimal detection of intracellular markers, such as cytokines and transcription factors. The dye is excited by the blue laser (488 nm), and its emission peak is at 514 nm. ... PerCP-Cy5.5: 490: 695: Laser and ... cvrsta stolica kod bebaWebPerCP-Cy5 is designed to be excited by the blue laser (488 nm) and detected using an optical filter centered near 680 nm (e.g., a 695/40 nm bandpass filter). The donor dye … cvrsta stolica kod odraslihWebAug 28, 2024 · What Colour is Cy5? Cy5 dye is a traditional far-red–fluorescent label for protein and nucleic acid conjugates used for imaging, flow cytometry, and genomic applications. Can you use Cy3 and Cy5 together? Cy3 dyes are yellow-orange. Specific filter wavelengths (550-600 nm and 655-695 nm) have to be used to avoid background … cvrstoca materijalaWebIn instances where only the antibody exhibiting non-specific binding (for example, CD3 APC/Cy7, CD56 PE/Cy5 and CD19 PE/Dazzle™ 594) was used to costain cells with CD14 BV421™, the True-Stain Monocyte … dji mavic 2 price in bangladesh