Nettet1. aug. 2009 · This method is based on the selective and reversible precipitation of ELP-tagged target proteins by gentle heating in the presence of high concentrations of sodium chloride. A critical aspect of this system is that the ELP tag is induced to self-cleave by a mild pH shift after purification. Nettetcleavage activity than the full-length intein (Fig. 1B, compare con-structs 1 and 3), while the ∆I-SM mutant behaved similarly to the full-length intein (Fig. 1B, compare constructs 1 and 4). A ...
Controllable protein cleavages through intein fragment …
Nettet12. jul. 2024 · In the present day, methanol is mainly produced from methane via reforming processes, but research focuses on alternative production routes. Herein, we present a chemo-/biocatalytic oxidation cascade as a novel process to currently available methods. Starting from synthetic biogas, in the first step methane was oxidized to formaldehyde … Nettet30. jan. 2024 · Inteins play important roles in the post-translational process with the ability of self-excision. It causes splicing of the flanking polypeptide sequences called external … detour snijeg znacenje
Intein Applications: From Protein Purification and Labeling to ...
Nettet5. des. 2013 · DOI: 10.4155/PBP.13.53 Corpus ID: 85097255; Intein-based purification tags in recombinant protein production and new methods for controlling self-cleavage @article{Shi2013InteinbasedPT, title={Intein-based purification tags in recombinant protein production and new methods for controlling self-cleavage}, author={Changhua Shi and … NettetProduction of antimicrobial peptide arasin-like Sp in Escherichia coli via an ELP-intein self-cleavage system. Journal of Biotechnology 2024, 347 , 49-55. ... Intein-based purification tags in recombinant protein production and new methods for controlling self-cleavage. Pharmaceutical Bioprocessing 2013, 1 (5) , ... Nettet21. aug. 2024 · Intein-mediated self-cleavages have been applied for developing purification tags because self-cleavable tags could eliminate proteolytic removal steps … detour prijevod na hrvatski