Paclitaxel resistance tubulin

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August undefined, 2024

WebAug 23, 2024 · The human ovarian cancer paclitaxel-resistant cell line A2780/Taxol was purchased from Shanghai Bogu Biotechnology Company; the drug resistance index was … WebThis effect was not significantly reduced in paclitaxel-resistant cells. Rhodamine 123 efflux assay showed that phentolamine was not a P-gp substrate. Phentolamine induced mitotic arrest of the cell cycle and formation of hyperdiploid cells, followed by an increase of apoptosis. ... similar to paclitaxel, induced tubulin polymerization and ...

Paclitaxel-resistant cells have a mutation in the paclitaxel-binding ...

WebFeb 11, 2010 · This partial restoration of paclitaxel sensitivity is related to the multifactorial nature of drug resistance and the fact that the paclitaxel-resistant NSCLC cells have other microtubule... Webcells selected for paclitaxel resistance [Schibler and Cabral, 1986], but to date only one a-tubulin mutation from a paclitaxel resistant cell line has been identiﬁed by sequencing … toaster and stove top

Paclitaxel-resistant Human Ovarian Cancer Cells …

WebGiven that the epithelial cancer cells lines incubated with CA-61 and -84 acquired a round-shaped morphology, which was similar to the cells treated with Vinblastine (Vin) and paclitaxel (PTX) , and taking into account their high affinity to the colchicine-binding site of tubulin, we sought to examine whether CAs interfered with tubulin ... WebMay 18, 2024 · The single channel tubulin and DAPI images that correspond to Figure 1, A–F, are shown in A and B. Loss of DTE in docetaxel-resistant PCa xenografts. To decipher in vivo mechanisms of … Webinvolving tubulin. We report two paclitaxel-resistant sublines derived from 1A9 human ovarian carcinoma cells. Single-step paclitaxel selection with verapamil yielded two clones that are resistant to paclitaxel and … toaster and sandwich maker

Direct Analysis of Tubulin Expression in Cancer Cell Lines by ...

Tubulin acetylation enhances lung cancer resistance to …

WebNov 11, 2024 · Paclitaxel is a chemotherapeutic agent that acts as an inhibitor of cellular mitosis and has been widely used in the treatment of triple-negative breast cancer … Webfrom parental and Taxol/epothilone-resistant human cancer cell lines. This method allows the direct determination of tubulin isotype composition, including post-translational modifications and mutations occurring throughout the entire protein. Four major isotypes, âI-,âIVb-, KR1-, andR6-tubulin, were detected in two human carcinoma cell lines ... toaster and sweeper light movieWebFeb 27, 2006 · Paclitaxel-resistant cells have a mutation in the paclitaxel-binding region of β-tubulin (Asp 26 Glu) and less stable microtubules Malathi Hari; Frank Loganzo; Tami Annable; Xingzhi Tan; Sylvia Musto; Daniel B. Morilla; James H. Nettles; James P. Snyder; Lee M. Greenberger Author & Article Information Mol Cancer Ther (2006) 5 (2): 270–278. toaster and kettle sets australia

"WebApr 17, 2001 · The chemotherapeutic drug Taxol is known to interact within a specific site on β-tubulin. Although the general location of the site has been defined by photoaffinity labeling and electron crystallography, the original data were insufficient to make an absolute determination of the bound conformation. " - Paclitaxel resistance tubulin

Paclitaxel resistance tubulin

Tubulin-Targeted Drug Action: Functional Significance of Class II …

WebThe cellular uptake, intracellular nanoparticle trajectory, and subsequent cellular events were evaluated after treatment with various PLGA nanocomplexes in human lung cancer A549 cells and A549-derived paclitaxel-resistant A549/T12 cell lines with -tubulin mutation.Results: A549 and A549/T12 cells contain constitutively activated Stat3, and ... Web58 rows · One study found that paclitaxel pre-treatment of their cisplatin-resistant cell lines increased the accumulation of cisplatin and was associated with tubulin alterations. 79 If …

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WebFeb 27, 2006 · Paclitaxel-resistant cells have a mutation in the paclitaxel-binding region of β-tubulin (Asp 26 Glu) and less stable microtubules Malathi Hari; Frank Loganzo; Tami … WebAug 23, 2024 · The human ovarian cancer paclitaxel-resistant cell line A2780/Taxol was purchased from Shanghai Bogu Biotechnology Company; the drug resistance index was 30, and the concentration of the culture solution to maintain paclitaxel resistance was 800 ng/mL). ... paclitaxel did not affect the expression of class III beta-tubulin. However, …

WebMar 19, 2024 · Microtubules are tiny, very rigid, cylindrical, hollow tubes with a diameter of approximately 25 nm, composed of the two globular proteins, α- and β-tubulin with a total molecular weight of 50 kDa each [Figure 1]. In humans, eight isoforms of each α- and β-tubulin have been identified[2]. WebMechanisms of the resistance to paclitaxel are complex as indicated by cumulative studies, which include alterations in microtubule dynamics, altered expression of β-tubulin isotypes, and also deregulated signaling pathways. 21–24 In our own serial studies, we have demonstrated that activation of PI-3K/Akt signaling plays a critical role in ...

WebGo analysis of the protein changes identified upon paclitaxel resistance revealed that cell component enrichment related to mitochondria. Moreover, tubulin and mitochondrial proteins were the major cellular components with changes associated with paclitaxel treatment. This suggests that mitochondria may play a role in paclitaxel resistance. WebIt has been approximately ten years since the Food and Drug Administration (FDA) approved paclitaxel for the treatment of platinum resistant epithelial ovarian carcinoma. …

WebApr 6, 2024 · Tubulin acetylation enhances lung cancer resistance to paclitaxel-induced cell death through Mcl-1 stabilization Tubulin acetylation enhances lung cancer …

WebFeb 11, 2010 · This partial restoration of paclitaxel sensitivity is related to the multifactorial nature of drug resistance and the fact that the paclitaxel-resistant NSCLC cells have … toaster and toaster oven combinationWebApr 14, 2024 · The role of miR-18a and increased autophagy is corroborated by another study that shows how paclitaxel-resistant MDAMB231 cells have higher basal levels of both miR-18a ... autophagy in breast cancer cells, it also leads to hyperacetylation of tubulin, thus hindering the fusion of lysosomes and autophagosomes, which results in … penn national claims dept phone numberWebDec 1, 2008 · Abstract. Aberrant expression of β-tubulin isotypes is frequently described in tumor tissues and tubulin-binding agent (TBA)–resistant cell lines. There is limited … toaster and toaster oven combination 31137WebJan 1, 2024 · While a variety of mechanisms have been linked to paclitaxel resistance, including overexpression of ATP-dependent translocase ABCB1 , mutations in drug binding sites on tubulin [2,3,4,5,6,7 ... toaster and toaster ovenWebFeb 28, 2024 · SB226 binds at the colchicine site in tubulin as confirmed by X-ray structure and surface plasmon resonance spectroscopy. • SB226 is highly potent and effectively … toaster and coffe maker in one outletWebClass III beta-tubulin (TUBB3) reduces microtubule stability and confers resistance to microtubule-stabilizing taxanes, including paclitaxel and docetaxel. Pancreatic ductal … toaster and toaster oven in oneWebinvolving tubulin. We report two paclitaxel-resistant sublines derived from 1A9 human ovarian carcinoma cells. Single-step paclitaxel selection with verapamil yielded two clones that are resistant to paclitaxel and … penn national communityy